| Plant Tissue Culture |
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Course Location and Contact Information

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| Course
Description
This course covers all aspects of micro-propagation techniques for producing high health native and exotic, rare and endangered plants.
The skills and knowledge gained will be especially useful to plant propagators and breeders, lab technicians, horticulturalists, botanists, nursery and allied trades
Students are encouraged to work on plant material with which they have a particular interest.
Topics covered:
- History and principles of Plant Tissue Culture
- Sources of contamination and aseptic techniques
- Surface sterilisation of different plant materials and introduction into the culture process
- Seed, spore, plant culture establishment
- Chemical composition and preparation of culture media
- Transfer and sub-culturing of explants into multiplication and rooting media
- Deflasking of tissue cultured plantlets
- Layout and operation of a basic plant tissue culture laboratory
- Equipment requirements and suppliers
- Research project on plant of choice
- Excursions to Plant Tissue Culture laboratories.
Plant Tissue Culture Media Release

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| Career Opportunities
Graduates will be equipped with the knowledge to set up a basic tissue culture laboratory at home and or to work in a tissue culture facility associated with plant nurseries
The skills and knowledge gained will also equip laboratory technicians to operate tissue culture facilities in schools and other plant science laboratories
Staff of plant nurseries will also be trained to deflask tissue-cultured plants obtained from tissue culture laboratories.
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| Course
Duration
Evening classes run from 5.30pm until 9.00pm (75 hours in total).
Excursion: 15 hours

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| Course
Commencement Dates
February /March.

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| Entrance
Requirements
Plant knowledge and or basic chemistry is recommended.

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| Application
Procedure
Apply directly to the Horticulture
Department

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| Skills Recognition
- Exemptions/Transfers
Students enrolling in this course who have completed studies at other institutions should discuss the possibility of being exempted from related units in this course. If a student can demonstrate a full understanding of the unit from their work, then they may discuss the option of being assessed for units in their workplace. Students are eligible for Skills
Recognition.

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| Pathways
to Further Study
These qualifications may lead to further study in the Certificate IV in Horticulture (Nursery) or other streams, and then into the Diploma of Horticulture (Nursery) or other streams.

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| Course Outline
NMIT, in consultation with industry, has constructed courses that satisfy these packaging rules and are tailored to the requirements of industry.
Units
RTF3014A Implement a propagation plan
RTF5012A Manage a controlled growing environment

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| Assessment
Methods
Performing aseptic techniques and surface sterilisation of plant materials, culture, media preparation, transfer of cultures and deflasking in the tissue culture laboratory and glasshouse.
Compulsory work for 30 hours researching and producing a crop of choice via micro propagation techniques

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| Materials
General laboratory and other tissue culture equipment. Students will be given more information when the course commences.

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| Qualification
These units are from the Certificate III in Horticulture and Diploma of Horticulture courses. Students will be issued with an in-house NMIT certificate after successful completion of the course.

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